| 2xYT Agar |
500g |
It is used for cultivating recombinant strains of Escherichia coli. Enzymatic Digest of Casein provides nitrogenous sources like amino acids and peptides. Yeast Extract supplies B-complex vitamins and minerals. Sodium Chloride maintains the osmotic balance. Agar is the gelifying agent. |
| 2XYT Microbial Medium |
500g |
It is a liquid medium used for cultivating recombinant strains of Escherichia coli. Enzymatic Digest of Casein provides nitrogenous sources like amino acids and peptides. Yeast Extract supplies B-complex vitamins and minerals. Sodium Chloride maintains the osmotic balance. |
| Agarose D-1 LE Low Electroendosmosis (EEO) |
500g |
It is specifically prepared for the use in electrophoretic studies to analyze DNA fragments from PCR products and digestion by restriction endonucleases, among others. Obtained from seaweeds, mostly Gelidium spp, agarose is a polymer composed of several agarobiose units that aggregate into a network with varying pore sizes. This property is used to separate nucleic acid fragments based on their lengths. Agarose is a gel at room temperature and remains firm at temperatures up to 65 ºC. In order to gelify, agarose must be dissolved, heated to its melting temperature (85-91ºC) and cooled down to 34-37ºC. |
| LB Agar (Lennox L Agar) |
500g |
It is a solid medium used for molecular genetic studies. Enzymatic Digest of Casein supplies amino acids and peptides. Yeast Extract is a source of B-complex vitamins and minerals. Sodium Chloride provides essential electrolytes and maintains the osmotic equilibrium. Agar is the gelifying agent. |
| LB Broth (Lennox LB Broth) |
500g |
It is a liquid medium used for the growth and maintenance of E. coli, being used in molecular microbiology processes. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation. Enzymatic Digest of Casein supplies amino acids and peptides. Yeast Extract is a source of B-complex vitamins and minerals. Sodium Chloride provides essential electrolytes and maintains the osmotic equilibrium. |
| Luria Agar (Miller’s LB Agar) |
500g |
It is used for maintaining Escherichia coli in molecular genetic studies. Enzymatic Digest of Casein supplies amino acids and peptides. Yeast Extract is a source of B-complex vitamins and minerals. Sodium Chloride provides essential electrolytes and maintains the osmotic equilibrium. Agar is the gelifying agent. |
| Luria Agar (Miller’s Modified) |
500g |
It is used for maintaining Escherichia coli in molecular genetic studies. Enzymatic Digest of Casein supplies amino acids and peptides. Yeast Extract is a source of B-complex vitamins and minerals. Sodium Chloride provides essential electrolytes and maintains the osmotic equilibrium. Agar is the gelifying agent. |
| M9 Minimal Salts |
500g |
It (with additions) is used to prepare M9 Minimal Medium, which is used to cultivate recombinant strains of Escherichia coli. M9 Minimal Salts is combined with a selected carbon and energy source (e.g. Dextrose) to create a minimally nutritious, chemically defined culture medium for recombinant Escherichia coli growth and culture maintenance. The ability to tightly control the nutritional content of the medium allows for the continued selection for specific plasmids encoding the ability to produce necessary vitamins and amino acids missing from the nutritional complement of the medium. By further supplementing the M9 Minimal Medium with selected amino acid or other necessary metabolites, it is possible to create a selection medium designed for specific auxotrophs, greatly expanding the flexibility of this medium to select for and support the growth of very unique recombinant Escherichia coli strains. |
| NZ Broth |
500g |
It is a nutrient-rich medium for the cultivation of lambda bacteriophage. Tryptone is a source of nitrogenous compounds. Sodium Chloride and Magnesium Sulfate provide essential electrolytes and maintain the osmotic equilibrium. |
| NZCYM Broth |
500g |
It is a liquid medium used for cultivating recombinant strains of Escherichia coli. Enzymatic Digest of Casein and Casamino Acids provide nitrogenous compounds. Yeast Extract is a source of B-complex vitamins. Sodium Chloride and Magnesium Sulfate provide essential electrolytes and maintain the osmotic equilibrium. |
| NZM Broth |
500g |
It is a liquid medium used for propagating bacteriophage lambda. Enzymatic Digest of Casein is a source of nitrogenous compounds. NZ-Amine A derives from the enzymatic digestion of casein and provides peptides, amino acids, and water-soluble vitamins. Sodium Chloride and Magnesium Sulfate provide essential electrolytes for enzymatic reactions (including DNA replication) and maintain the osmotic equilibrium. |
| NZYM Broth |
500g |
It is a liquid medium used for propagating bacteriophage lambda. Enzymatic Digest of Casein is a source of nitrogenous compounds. NZ-Amine A derives from the enzymatic digestion of casein and provides peptides, amino acids, and water-soluble vitamins. Yeast Extract supplies B-complex vitamins. Sodium Chloride and Magnesium Sulfate provide essential electrolytes for enzymatic reactions (including DNA replication) and maintain the osmotic equilibrium. |
| RM Agar Base Medium |
500g |
It is used for the maintenance and propagation of the promoter PL in E. coli strains and to increase the yield of plasmid for sequencing positive clones. These strains have the gene Lambda cI repressor, under the control of the promoter tryptophane inducible, trp. This medium has low tryptophane levels. |
| SOB Medium (Optimal Broth or Hanahan’s Broth) |
500g |
It is recommended for the cultivation of recombinant strains of E. coli. Enzymatic Digest of Casein provides nitrogenous compounds, while Yeast Extract is a source of B-complex vitamins. Sodium Chloride, Potassium Chloride, and Magnesium Sulfate provide essential electrolytes and maintain the osmotic balance. |
| Terrific Broth (TB) |
500g |
It is a medium used with Glycerol in cultivating recombinant strains of Escherichia coli. Enzymatic Digest of Casein provides nitrogenous compounds and minerals, while Yeast Extract is a source of B-complex vitamins. Mono- and Dipotassium Phosphates serve as pH buffers. |
| Terrific Broth, Modified |
500g |
It is a medium used with Glycerol in cultivating recombinant strains of Escherichia coli. Although the bacteria are slower growing in this medium, the intention of Terrific Broth, Modified is to improve plasmid yields and cell density. Tryptone provides nitrogenous compounds and minerals, while Yeast Extract is a source of B-complex vitamins. Mono- and Dipotassium Phosphates serve as pH buffers. |
| Yeast Nitrogen Base |
500g |
It is based on the formula designed by Wickerham and recommended for the classification of yeast strains based on their ability to assimilate carbon compounds. The addition of carbon sources allows for its use in susceptibility testing with anti-fungal drugs when defined liquid medium is needed. |
| Yeast Nitrogen Base w/o AA & Ammonium Sulfate |
500g |
It is based on the formula designed by Wickerham and recommended for the classification of yeast strains based on their ability to assimilate amino acids and carbon compounds. The broth medium contains all the essential vitamins and inorganic salts necessary for the cultivation of yeasts, except for histidine, methionine, tryptophane and a carbohydrate source. |
| Yeast Nitrogen Base w/o Amino Acid |
500g |
It is based on the formula designed by Wickerham and recommended for the classification of yeast strains based on their ability to assimilate amino acids and carbon compounds. The broth medium contains all the essential vitamins and inorganic salts necessary for the cultivation of yeasts, except for histidine, methionine, tryptophane and a carbohydrate source. |
| YP (Yeast Peptone) Agar Base |
500g |
It is a solid medium for the cultivation of yeast in molecular microbiology procedures. Peptone and Yeast Extract provide all the nutrients required for yeast growth: amino acids, peptides, carbohydrates, vitamins, and minerals. Agar is the gelifying agent. |
| YP Base Media |
500g |
YP (Yeast Peptone) Base Media is a medium for the cultivation of yeast in molecular microbiology procedures. Peptone and Yeast Extract provide all the nutrients required for yeast growth: amino acids, peptides, carbohydrates, vitamins, and minerals. |
| YPD Agar |
500g |
It is a solid medium for the maintenance and propagation of yeast in molecular microbiology procedures. Enzymatic Digest of Casein provides nitrogenous compounds, vitamins, and trace elements. Dextrose is the fermentable carbohydrate. Agar is the gelifying agent. |